【摘要】 监测金黄色葡萄球菌的耐药状况 ,为临床治疗耐甲氧西林金黄色葡萄球菌 (MRSA)的感染提供依据。采用试管双倍稀释法 (改良 )及纸片扩散法对 12 8株金黄色葡萄球菌进行药敏试验 ,并用 Nitrocefin纸片法测定其 β-内酰胺酶。 MRSA检出率 72 .6 5 % ,产 β-内酰胺酶 83.70 %。对青霉素 G、克林霉素、红霉素、头孢唑啉、庆大霉素、四环素的耐药率在 10 0 %～ 92 .47%之间 ,万古霉素、去甲万古霉素对所有菌株的抑菌率达 10 0 %。多数药物对甲氧西林敏感金黄色葡萄球菌 (MSSA)作用良好 ,头孢唑啉、利福平、亚胺培南和异帕米星的抑菌率均在 94.3%～ 10 0 %之间。MRSA对多数 β-内酰胺类、氟喹诺酮类及某些氨基糖苷类抗生素呈多重耐药性。万古霉素、去甲万古霉素对其显示最强的抗菌活性 ,利福平、壁霉素、异帕米星亦显示较强的抗菌活性 ,提示上述抗菌药物可用于 MRSA所致严重感染更多还原

【Abstract】 In order to control the nosocomial infections caused by methicillin resistant staphylococcus aureus (MRSA), the resistance of MRSA isolates from patients was investigated, the susceptibility to 24 antibacterial agents tested by using agar dilution test and Kirby Bauer respectively, and the β lactates deteted by using nitrocefin method. The results showed the detectable rate of MRSA was 72 65 % 83 7 % MRSA were positive for β lactamase. The resistance rate of PG, clindamycin, erythromycin. Cefazolin, gentamicin, tetracycline was from 92 24 % to 100 %. Vancomycin and norvancomycin could inhibit all isolates tested. Most of the drugs were active against methicillin sensitive staphylococcus. The inhibition rate of cefazolin, rifampin, imipenem and isepamicin was from 94 3 % to 100 % MRSA was multidrug resistance to most of β lactams fluoroquiolones and some aminoglycoside antibiotics. Vancomycin and norvancomycin possessed a strongest activity for MRSA, and rifampin, teicoplanin and isepamicin also revealed a stronger active against MRSA, suggesting all of them could be used to control MRSA infection.更多还原