【摘要】 采用日本血吸虫重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗免疫ＢＡＬＢ／ｃ小鼠。免疫８周时，断头取血，取脾脏，取腹腔巨噬细胞。以淋巴细胞刺激指数（ＳＩ）反映细胞增殖能力，以ＮＯ释放量检测巨噬细胞吞噬活性，以ＥＬＩＳＡ试剂盒检测血清及脾淋巴细胞培养上清的白细胞介素２（ＩＬ－２）和干扰素－γ（ＩＦＮ－γ）的含量。结果表明，ｒＢＣＧ－Ｓｊ２６ＧＳＴ疫苗以 １０~６ＣＦＵ剂量经皮下免疫小鼠后，小鼠脾淋巴细胞增殖能力明显高于对照组、载体组和ＢＣＧ组（均为Ｐ＜０．０５）；小鼠腹腔巨噬细胞ＮＯ的含量明显高于对照组和载体组（均为Ｐ＜０．０１）；小鼠血清ＩＬ－２的含量明显高于对照组（Ｐ＜０．０１）和载体组（Ｐ＜０．０５）；而小鼠脾淋巴细胞培养上清ＩＬ－２的含量分别高于对照组４４％、载体组４８％和ＢＣＢ组４２％；小鼠血清ＩＦＮ－γ的含量分别高于对照组２０％、载体组１９％和ＢＣＧ组１３％，均有升高趋势、结果提示，ｒＢＣＧ－Ｓｊ２６ＧＳＴ疫苗能明显增强小鼠的免疫应答反应，其抗感染作用一方面与ＢＣＧ本身的佐剂特性有关，另一方面与机体产生抗Ｓｊ２６－ＧＳＴ特异性抗体有关。更多还原

【Abstract】 In order to investigate the immunogenicity of recombinant BCG bearing Shistosoma japon- icum 26 ku glutathione S-transferase gene (rBCG -Sj26GST), the specific pathogen-free BALB/c mice were immunized with rBCG-Sj26GST vaccine in dose of 10~6 CFU by SC. The lymphocyte stimulating index (SI), macrophage activity as well as IL-2, IFN-γ levels of the serum and culture supernatant of spleen lymphocytes were tested in the killed mice on the 8th week of inoculation. The results showed that SI was significantly higher than those in the control, vector and BCG groups (P<0. 05). The macrophage activity was significantly higher than in the control and vector groups (P<0. 01). The serum IL-2 level was significantly higher than in the control group (P<0. 01) and vector group (P<0. 05). Compared with control, vector and BCG groups, the IL-2 level of the culture supernatant of spleen lymphocytes was increased by 44%, 48% and 42% respectively, and the serum IFN-γ level by 20%, 19% and 13% respectively. These findings suggest that the rBCG-Sj26GST vaccine could obviously enhance the immune response of the BALB/c mice, which is related with the immun adjuvant activity of BCG, on the other hand with specific anti-Sj26GST antibody from BALB/c mice.更多还原