【摘要】 目的 :测定复方羌芪水提液的体外抗 CVB3作用 ,并对其抗病毒机理进行初步的探讨。方法 :用含不同浓度复方羌芪水提液的维持液培养 CVB3感染的 Vero细胞 ,48h后用 MTT法测定细胞活性 ;培养上清液滴定 TCID50 。结果 :复方羌芪水提液能抑制 CVB3对 Vero细胞的致病变作用 (CPE) ,使细胞存活率升高 ,培养上清病毒滴度降低 ;且随药物浓度及药物与病毒作用时间的延长抑制作用增强。结论 :复方羌芪水提液的抗病毒机制可能是直接杀伤病毒及影响病毒在细胞内的生物合成 ,而不是影响病毒吸附和穿入环节更多还原

【Abstract】 Objective:The compounded Qiangqi extract was used to investigate its antiviral activity in Vero cells as a specific inhibitor of Coxsackievirus B 3 (CVB 3) replication, and its antiviral mechanism was exploredMethods:Vero cells infected by CVB 3 were cultured with different concentration of the compounded Qiangqi extract for 48 hours, and its inhibitory effect on CVB 3 replication was evaluated with cell survival rates by MTT assay and 50% tissue culture infection dose (TCID 50 ) of culture supernatantResults: The compounded Qiangqi extract could significantly inhibit cytopathic effect (CPE) of CVB 3-infected Vero cells, increase survival rates of CVB 3infected cells in dose dependent manner and decrease viral titres of culture supernatantConclusion:It may be the antiviral mechanism of the compounded Qiangqi extract that it may kill viruses directly and inhibit the replication of CVB 3 in cells, without preventing from viral adsorption and penetration更多还原