【摘要】 采用含有甜菜坏死黄脉病毒外壳蛋白 (BNYVVCP)基因的农杆菌 ,分别以不同甜菜品系的叶柄、下胚轴及子叶为外植体材料进行基因转化研究。含有BNYVVCP基因和卡那霉素抗性筛选基因 (Kanr)的农杆菌与甜菜组织不同外植体共培养后 ,经过诱导分化培养 ,在含有卡那霉素 (kanamycin)的培养基上 ,从叶柄及下胚轴分别直接诱导出抗卡那霉素再生芽 ,而从子叶上只诱导出紧密型绿色愈伤组织 ,未分化出不定芽。从叶柄及下胚轴诱导再生芽的诱导培养基分别为 :MS +BA0 5+TIBA0 2 +3 %蔗糖 +Kan2 0 0 +Cb50 0、MS+BA0 1 5+NAA0 2 +3 %蔗糖 +Kan1 0 0 +Cb50 0。甜菜再生植株诱导频率与甜菜品系、外植体类型、苗龄、温度、TIBA、预处理等因素有关。再生植株生根培养基为 :MS +IBA2 0 +2 %蔗糖 +Kan50。更多还原

【Abstract】 The regeneration and transformation of sugar beet using Agrobacterium tumfaciens-mediated method and a kanamycin-risistance selection system were studied in this paper. Different explants derived from petiole, hypocoty1 and cotyledon were inoculated with A. Tumefaciens harbouring BNYVV CP gene and Kan r gene. Inocalated tissues were cultured on shoot inducing Medium. In medium MG1, kanamycin-resistance regeneration soots was obtained from petiole; in medium MG2, Kanamycin-resistance regeneration shoots were obtained from hypocoty1. Only firm green callus were initiated from cotyledons. Medium MG1 was Ms+BA0 5+TIBA0 2+Kan200+Cb500+3% Sucrose, medium MG2 was MS+BA0 5+NAA0 2+Kan50+Cb500+3% Sucrose. Shoot regeneration frequency depended on the genotypes, original explant, seedling age, culturing temperature, hormone concentration TIBA treatment. The rooting medium was MS+IBA2 5+Kan 50+2% Sucrose.更多还原