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超低温冻存前后胎儿脐血中NK细胞活性的研究
Experimental Study on Natural Killer Cells Activity of Fetal Cord Blood Pre- and Post- frozen
【摘要】 目的 探讨影响胎儿脐血NK细胞活性的因素。方法 采用台盼蓝染色法检测NK细胞活性 ,脐血MNC用Ficoll分离液离心获得。结果 经液氮 (- 196℃ )冻存后脐血NK活性比冻存前显著提高。结论 提示超低温能解除抑制脐血NK细胞活性的因素
【Abstract】 Objective To investigate factors that affect NK cells activity of fetal cord blood.Methods MNCs were isolated by Ficoll seperation,and NK cells activity is assayed with Trypanblue dying.Results NK cells activity of ten fetal cord blood frozen with liquid-nitrogen increased significantly compared with that pre-frozen.Conclusion It suggests that deep-frozen may relieve effect of depression factors on cord blood NK cells activity.
- 【文献出处】 苏州医学院学报 ,ACTA ACADEMIAE MEDICINAE SUZHOU , 编辑部邮箱 ,2000年04期
- 【分类号】R318.52
- 【下载频次】127