【摘要】 目的　分析抗B7- 1功能性单克隆抗体对表达B7分子的人恶性淋巴瘤细胞株Raji和Daudi细胞体外生长的抑制作用及其可能机制。方法　采用免疫荧光标记和流式细胞仪分析肿瘤细胞的B7- 1分子表达 ;以不同浓度抗B7- 1功能性单克隆抗体 ,加入体外培养细胞中 ,经细胞计数和MTT法 ,观察单克隆抗体对肿瘤细胞体外生长的作用。结果　Raji和Daudi细胞均表达B7- 1分子 ,表达阳性百分率分别为 92 .7%和 89.2 % ;抗B7- 1单克隆抗体浓度在 5 μg/ml时就对这两种细胞株有显著的抑制作用 ,并随浓度增高而增强。 结论　抗B7- 1单克隆抗体对高表达B7- 1分子的人恶性淋巴瘤细胞体外生长有明显的抑制作用 ,这种抑制作用是通过诱导肿瘤细胞的凋亡所致更多还原

【Abstract】 Objective To investigate the effects of mouse anti-human B7-1 monoclone antibody (McAb) on the growth of B7-1 expressing Raji and Daudi cells.Methods The expression of B7-1 molecule on Raji and Daudi cells was studied by using immunofluorescence and FCM analysis;Raji and Daudi cells were cultrued with different concentration of anti-human B7-1 McAb,then the inhibitory effects of McAb on the growth of Raji and Daudi cells were observed by cellular counting and MTT assay.Results Both Raji and Daudi cells expressed B7-1 molecule,and the percentage of positive result was 92.7% and 89.2% respectively.When the anti-human B7-1 McAb concentration was at 5.0μg/ml,it remarkably inhibited the growth of Raji and Daudi cells.Conclusion Anti-human B7-1 McAb obtained in our laboratory can obviously inhibit the growth of Raji and Daudi cells,and this functional anti-B7-1 McAb may have a potential value for clinic application.更多还原