节点文献
α2a或α2b基因工程干扰素对CD4+/CD8+的比值和NK活性的影响
The Effect of Recombinant IFNα2a or IFNα2b on CD4+/CD8+ Propotion and NK Activity
【摘要】 目的 研究不同品牌IFNα2a或IFNα2b的免疫调节作用。方法 采用MTT法进行NK细胞的细胞毒试验和用流式细胞仪测定淋巴细胞CD抗原表达。结果 2 0 0IU/ml不同品牌的IFNα2a或IFNα2b能促进NK细胞杀伤K562 细胞 ,其杀伤效率可达 35 %左右 ,比不加干扰素对照组NK细胞杀伤效率约 14%均提高了近 2 .5倍 (P <0 .0 5 ) ,可使CD4 + /CD8+ 的比值升高 (P <0 .0 5 )。结论 进口和国产的不同品牌IFNα2a和IFNα2b具有同等的免疫调节功效。
【Abstract】 Purpose To study the immune regulation of IFNα 2a or IFNα 2b from various manufatories.Method The cytotoxicity of NK was measured by MTT assay and the express of CD antigen on lymphocytes examined by FACS.Results All the IFNα 2a or IFNα 2b from different manufactories enhanced NK cytotoxicity with the killing efficiency (about 35%) which was 2.5 times higher than that shown in the control group without IFNα (about 14%);moreover,they decreased the CD 8 + lymphocytes and CD 4 +/CD 8 + proportion.Conclusion The imported and domestic IFNα 2a and IFNα 2b possess the similar immunoregulative function.
【Key words】 recombinant interfron； nature killing efficiency； CD antigen； immune regulation；
- 【文献出处】 苏州医学院学报 ,ACTA ACADEMIAE MEDICINAE SUZHOU , 编辑部邮箱 ,2000年03期
- 【分类号】R392-33
- 【被引频次】1
- 【下载频次】40