【摘要】 以新铁炮百合叶片、花瓣、花丝、鳞片和种子为外植体，进行组织培养再生研究。结果表明：不同外植体诱导分化的能力不同，其中种子＞鳞片＞花丝＞花瓣＞叶片，诱导率分别为９８．２７％，９５．８３％，８６．５０％，１４．６３％和１１．４３％。不同继代培养基对新铁炮离休快繁有显著影响，选用ＭＳ作为基本培养基，附加不同浓度的ＢＡ和ＮＡＡ配合成１０个组合，并进行随机区组实验，结果表明：ＭＳ＋ＢＡ２ｍｇ·Ｌ－１＋ＮＡＡ０．２ｍｇ·Ｌ－１继代繁殖效果最佳，繁殖倍数４０ｄ达１４～１７株苗。不同生根培养基对新铁炮百合继代苗生根影响差异显著，其中以ＭＳ不附加任何激素为最佳，生根率为９９％，平均每株苗生粗根６．９条。不同移栽基质对新铁炮百合生根苗移栽成活率影响不同，其中以细河沙：草炭（２：１）为最佳，移栽成活率可达９２％。更多还原

【Abstract】 Using the leaves, petals, filaments, scales and seeds as the explant, the regeneration of insure culture of Lilium×longiflorum was studied. The result indicates that the difference of the bud initiation was obvious on the vitro various explant. The seeds, scales and filaments had high frequency of bud-inducing, with 98.27%, 95.83% and 86.54% respectively. According to the test of statistics random block, MS medium supplemented with various combinations of plat grouch regulators effected the sprouting of the bud seedings. MS plus BA 2mg.L-1 and NAA 0.2mg.L-1 are the best of 10 combinations of difference hormone. It produced 14 indefinite bud seedings by 1 and in 30-40 days. MS medium supplemented no hormone Produed the rooting frequency of 99.0% and 6.9 average roots in one indefinite bud seeding. When the rooted seedings were transplanted in the greenhouse, the survival rate reached as high as 92% in the mixed medium of sand and peat (2: 1).更多还原