【摘要】 目的　研制一种高效广谱的新型钩体疫苗。方法　用PCR方法扩增致病性钩端螺旋体赖型 0 17株外膜蛋白OmpL1基因 ,将其克隆入大肠杆菌—分枝杆菌穿梭质粒载体pMV2 6 1和pMV36 1中。结果　筛选到两个重组载体pBQ1和pBQ2 ,通过电穿孔导入卡介菌 ,重组体经诱导表达了相对分子质量约为 35 0 0 0的融合蛋白。结论　卡介菌能有效表达钩体抗原蛋白更多还原

【Abstract】 Objective To prepare a new broad spectrum leptospira vaccine. Methods OmpL1 gene was amplified from the genome of leptospira serovar Lai Strain 017 by PCR and cloned into E.coli BCG shuttle plasmids pMV261 and pMV361. Results Two recombinant vectors pBQ1 and pBQ2 were constructed and transformed to BCG by electroporation. The fusion protein with a molecular weight of 35 000 was expressed under the induction of high temperature (45℃).Conclusion Leptospira antigen protein can be effectively expressed in BCG.更多还原