【摘要】 目的　对７份ＨＢｓＡｇ诊断试剂检测结果不一致的样品进行确证并分析其原因。方法采用套式 ＰＣＲ法对７份样品进行ＤＮＡ扩增，并将阳性产物克隆到ｐＧＥＭ－Ｔｅａｓｙ载体上，然后进行测序并对其序列进行分析。 结果　７份样品中６份为ＨＢＶ　ＤＮＡ阳性，其中５份与Ａｂｏｏｔｔ和Ｏｒｔｈｒｏ　ＨＢｓＡｇ试剂无反应或反应较弱，而且其ａ决定 簇的氨基酸均在１３４位发生改变，由Ｆ变为 Ａ；１份与 Ａｂｂｏｔｔ和Ｏｒｔｈｒｏ ＨＢｓＡｇ试剂反应较强，其ａ决定簇的氨基酸也 在１３４位发生改变，但由Ｆ变为Ｓ。结论　国内外首次报道ＨＢｓＡｇ ａ决定簇１３４位由 Ｆ变为 Ａ后，其抗原性明显降 低，不易被ＨＢｓＡｇ试剂检出；而１３４位由对变为 Ｓ后，其抗原性无明显改变。更多还原

【Abstract】 Objective To analyze the cause of inconsistent detection results of 7 specimens by diagnostic kit for HBsAg and further identify the sqecimens.Methods The DNA sequences of the 7 specimens was amplified by nested PGR, then the positive products were cloned into pGEM-T easy vector and sequenced. Results HBV DNA were detected out in 6 of the 7 specimens,and 5 of the 6 specimens showed no or weak reactions with Abbott and Orthro HBsAg kits. All the amino acids of antigen determinant ’a’ of the 5 specimens changed from F to A at residue 134. However,the rest one showed strong reaction with Abbott and Orthro HBsAg kits,and the amino acid of antigen determinant ’a’ of it changed for F to S at the same site. Conclusion After the amino acid of antigen determinant’a’ changed from F to A at residue 134, the antigenicity of HBsAg was significantly decreased and not easy to be detected out by HBsAg kit; however,after the amino acid changed from F to S, no significant change of antigenicity was observed.更多还原