【摘要】 建立一种快速、准确测定各种豆类试样及粗蛋白质中的色氨酸含量的分光光度法讨论最佳试验条件及试剂用量 ,反应物在 4 1 0nm处具有最大吸收波长ε =1 1 0 3× 1 0 4 L·mol- 1·cm- 1,回收率 93 50—1 1 0 2 0 % ,相对标准偏差RSD <2 61。更多还原

【Abstract】 A spectrophometric method has bee developed for a rapid and accurate determination of tryptophan in a variety foods sample and coarse Protein.The optimal condition and reagents added are discussed.The golden yellow color complex is formed and maximum absorbance is obtained at 410nm with maximum molar absorptivity of 1.103X10 4 L·mole -1 ·cm -1 .The recovery rate and between 93.5-110.0%.RSD<2.61.更多还原