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联合固氮菌Alcaligen faecalis泌铵突变体离子束选育及鉴定

Screening and characterization of NH4+-excreting mutants of Alcaligen faecelis induce by ion inplantation

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【作者】 涂友斌王纪姚建明

【Author】 TU You-bin 1,WANG Ji 2,YAO Jian-ming 2 (1 Biochem.Dept.,SuZhou University,Suzhou,215007,China; 2 Centre of Ion Beam Bioengineering.Institute of Plasma Physics,Academia Sinica,Hefei,230031,China)

【机构】 苏州大学基础部生化室!苏州215007中国科学院等离子体物理所离子束生物中心!合肥230031

【摘要】 N+注入野生型菌株 (Alcaligenfaecalis 1 .4 88) ,筛选出抗 51 6mmol/L乙二胺的泌铵突变株EM1 1 0 5,它以KNO3为氮源时 ,培养 2 1h泌铵量可达到 1 1 0mmol/L。研究了该突变株在不同氮源下的培养特性 ,推断为铵载体缺陷型。

【Abstract】 NH 4 +-excreting mutant was isolated from Alicaligen faecelis 1.488 as ethyldiamine resistant strains which were obtained by mutagenesis by ion implantation.The mutant EM1105 could excrete up to 1.10mmol/L ammonium,while incubated in media containing KNO 3 as the sole nitrogen source during non-diazotrophic growth.After a study of properties of EM1105 incubated in media containing different nitrogen source,we can infer,the mutant EM1105 may be a NH 4 +-carrier deficient mutant.

  • 【文献出处】 生物学杂志 ,Joural of Biology , 编辑部邮箱 ,2000年05期
  • 【被引频次】9
  • 【下载频次】61
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