节点文献
光合细菌Chromatium vinosum可溶性氢酶的分离提纯及特性
Purification and Properties of Soluble Hydrogenase from Photosynthetic Bacterium Chromatium vinosum
【摘要】 光合细菌 Chromatium vinosum可溶性氢酶经 4次柱层析 (Whatman DE- 52 ,TSK- DEAE,Ultragel Ac A- 44 ,Mono Q)分离提纯后被纯化 630倍 ,得率为 33.5% .可溶性氢酶催化放氢比活性为 7.5μmol· min-1· mg-1.SDS- PAGE结果显示 ,可溶性氢酶由 52 k D和 2 1 .5k D两个亚基组成 .大亚基 N端氨基酸序列为 :SRTITIEPVTRXEGHAR;小亚基 N端氨基酸序列为 :STQPKIT-VATXLDG.氧化态可溶性氢酶在 54K时产生了典型的 Ni( )电子顺磁共振 (EPR)信号 (gxyz=2 .37、2 .1 6、2 .0 1 6和 gxyz=2 .30、2 .2 3、2 .0 1 6) .研究结果表明 ,C.vinosum可溶性氢酶是一种新的催化放氢的 Ni Fe-氢酶 .
【Abstract】 The soluble hydrogenase(SH)from Chromatium vinosum was purified 630 folds with a yield of 33.5% and a specific activity of 7.5 μmol·min -1 ·mg -1 by 4 steps of column chromatography(Whatman DE\|52,TSK\|DEAE,Ultragel AcA\|44 and Mono Q).The enzyme consists of two alien subunits(52 kD,21.5 kD).The N\|terminal sequence of the large subunit and small subunit were SRTITIEPVTRXEGHAR and STQPKITVATXLDG respectively.The oxidized soluble hydrogenase exhibited a typical Ni(Ⅲ) EPR(electron paramagnetic resonance)signals( g x,y,z =2.37,2.16,2.016 and g x,y,z =2.30,2.23,2.016).The results showed that the SH from C.vinosum was a new NiFe\|hydrogenase which catalyze H 2\|production.
【Key words】 Chromatium vinosum; Soluble hydrogenase; Purification and properties;
- 【文献出处】 中国生物化学与分子生物学报 ,Chinese Journal of Biochemistry and Molecular Biology , 编辑部邮箱 ,2000年06期
- 【被引频次】3
- 【下载频次】131