节点文献
用PCR及RFLP方法分析支原体与植原体的同源性
A homologous comparison between mycoplasma and phytoplasma using 16S rDNA PCR am plification and RFLP analysis
【摘要】 将猪鼻支原体和泡桐丛枝病植原体的16S rDNA进行PCR扩增,分别得到一条1 kb左右的扩增片段。PCR扩增产物用限制性内切酶EcoRⅠ、HindⅢ、BamHⅠ、SalⅠ和SmaⅠ进行RFLP(限制性片段长度多态性)分析,发现用RFLP分析猪鼻支原体和泡桐丛枝病植原体16S rDNA序列同源性的相关系数为072。
【Abstract】 16S rDNA from mycoplasma and phytoplasma was amplified by PCR, and a band was de tected at about 1 kb. DNA products were digested with restriction enzymes Eco RⅠ,HindⅢ,BamHⅠ,SalⅠ and SmaⅠ, and then were analyzed usi ng RFLP. Similarity coefficient derived from RFLP analysis of mycoplasma and phy toplasma is 0.72.
【基金】 1995年中国科学院生物区系分类资助课题!(960211)
- 【文献出处】 生物多样性 ,CHINESE BIODIVERSITY , 编辑部邮箱 ,2000年01期
- 【分类号】Q503
- 【被引频次】6
- 【下载频次】115