微生物蛋白酶对玉米皮蛋白水解作用的研究

【摘要】 研究了 1398中性蛋白酶对玉米皮蛋白的水解效果 ,并研究了酶用量、底物浓度、水解时间对该酶水解效果的影响。结果表明 ,最佳工艺条件为 :温度 40℃、p H7.5时 ,底物浓度 10 % ,酶用量 30 0 0 u/g,水解时间为 3h时 ,蛋白质溶出率最高达 90 .30 %更多还原

【Abstract】 Hydrolysis effects of 1398 proteinases on cornbran protein was studied.Effects of influencing parameters,including enzyme does,substrate concentration and hydrolytic time on hydrolysis of Proteinases was also investigated.Results indicate that the best combination of parameters for 1398 Proteinases in 40℃,pH 7.5,10% substrate, 3000 u/g enzyme and 3h.In such conditions 90.30% of the protein in cornbran was hydrolyzed.更多还原