【摘要】 将疫苗包裹在可生物降解的微球中 ,是一种极有潜力的新型疫苗载体系统 .用聚 -DL-乳酸 -聚乙二醇共聚物 ( PEL A)为材料 ,包裹乙型肝炎表面抗原 ( HBs Ag) ,制成缓释微球疫苗 ,以皮下注射或口服的方式免疫 BAL B/c小鼠 ,研究其免疫原性 .同时以乙肝常规铝佐剂疫苗免疫两剂作为对照 .结果表明 ,皮下注射单剂微球疫苗后 ,在第 1 4周 ,小鼠血清 Ig G滴度可达到与铝佐剂疫苗组相当的水平 ,维持较高的滴度 ;此外 ,口服微球疫苗组诱导的血清 Ig G滴度较低 ,但其 SIg A明显高于对照组和皮下注射微球疫苗组 ,可见 ,口服微球疫苗可诱导更高水平的粘膜抗体反应 .PELA微球作为疫苗载体系统是可行的 ,为研制新型疫苗提供了一条途径 .更多还原

【Abstract】 Encapsulation of vaccines in biodegradable microspheres provides excellent vaccine delivery system.Microspheres prepared from poly dl lactide poly (ethylene glycol,PELA) using a solvent evaporation process are used for encapsulating Hepatitis B surface antigen (HBsAg). Encapsulated HBsAg was administered to BALB/c mice by subcutaneous injection or per os to assess its immunogenicity . The control group received two injections of 10 μg HBsAg on alum at 0 and 4 weeks. Results indicated that a single injection of HBsAg microspheres could induce the same antibody level comparable to the two injection alum absorbed HBsAg at 14th week. On the other hand, oral administration stimulat ed lower serum IgG titer than the control group,but its SIgA titers from the salivary were higher than that of control groups and the subcutaneous group. These preliminary results suggest that biodegradable vaccine could be used as a potent HBsAg delivery system in inducing immune response. However, the condition of preparation and the immune effect of the vaccine should be further improved.更多还原