【摘要】 杀虫晶体蛋白是苏云金芽孢杆菌主要杀虫成分；进一步提高杀虫晶体蛋白的表达量是苏云金芽孢杆菌病效工程菌构建的主要途径。本文讨论了cry基因启动子活性、mRNA稳定性、不同cry基因间的协同表达以及伴孢晶体的形成等几个方面在转录水平或转录后水平上对杀虫晶体蛋白表达的影响。更多还原

【Abstract】 Insecticidal crystal proteins (ICPs) are the main effective component of Bacillus thuringiensis (Bt) against pests. The improvement of ICPs’ expression is a widely adopted way in the construction of Bt engineered strains. In this paper, we discussed the mechanism of ICPssuperexpression at transcription and post-transcription levels covering the activity of cry gene promoter, mRNA stability coexpression of different cry genes and crystal formation.更多还原