【摘要】 本研究观察了钙调神经磷酸酶 (CaN)在血管紧张素Ⅱ (AngⅡ )刺激的大鼠心脏成纤维细胞增殖中的作用。在培养的大鼠心脏成纤维细胞上 ,应用双波长荧光光度计检测Fura 2标记的细胞游离Ca2 +浓度 ;应用对硝基苯磷酸 (PNPP)作底物测定钙调神经磷酸酶 (CaN)活性 ;根据 3H 胸腺嘧啶掺入法评估CaN特异性抑制剂环胞素A(CsA)对AngⅡ刺激的心脏成纤维细胞DNA合成的影响。结果表明 ,AngⅡ (10 -7mol/L)刺激培养的大鼠心脏成纤维细胞 3H 胸腺嘧啶掺入较对照组增高 72 % (P <0 0 1) ,CsA可以抑制AngⅡ刺激的细胞 3H 胸腺嘧啶掺入。AngⅡ刺激的大鼠心脏成纤维细胞胞内Ca2 +浓度及CaN活性较对照组分别增高 112 % (P <0 0 1)和 17% (P <0 0 5 )。结果提示 ,CaN在一定程度上参与AngⅡ诱导的心脏成纤维细胞增殖的信号传递。更多还原

【Abstract】 The present study was undertaken to observe the role of calcineurin (CaN) in the angiotensinⅡ (AngⅡ) stimulated cardiac fibroblast proliferation. In cultured cardiac fibroblasts of neonatal rats, AngⅡ was used to stimulate proliferation while CaN was inhibited by CaN CsA inhibitor cyclosporin A (CsA). ~ 3H TdR incorporation, activity of CaN and intracellular calcium concentration were measured. ~ 3H TdR incorporation of AngⅡ stimulated fibroblasts was 72% higher than control ( P <0 01), which was inhibited by CsA (0 1～10 μmol/L) in a dose dependent manner. Intracellular Ca 2+ level and CaN activity of AngⅡ stimulated fibroblasts were respectively 112% ( P <0 01) and 17%( P <0 05) higher than control. It is concluded that CaN may play an important role in signal transduction of the AngⅡ induced cardiac fibroblast proliferation.更多还原