【摘要】 从雄性初断乳SD大鼠肝匀浆中提取肝刺激因子 (HSS)并加以部分纯化 ,观察其促人肝癌细胞增殖活性及对p2 1ras蛋白表达的影响。结果表明 :(1)HSS具有明显的促人肝癌细胞增殖活性 ,其分子量为 14～ 2 0kD ;(2 )HSS可提高p2 1ras蛋白表达 ,具有时间 效应关系 ,并与EGF呈协同作用 ;(3)HSS调节p2 1ras蛋白表达具有剂量 效应关系 ,且呈现出饱和性。鉴于我们已报道HSS上调EGF受体蛋白和基因表达这一事实 ,本实验结果进一步说明 ,HSS促人肝癌肝细胞增殖与其调节EGF受体介导的信号分子传导过程相关。更多还原

【Abstract】 The hepatic stimulator substance (HSS) was partially purified from schedule lighted weanling rat livers. The effects of HSS on p21 ras expression of human hepatic carcinoma cell BEL 7402 were examined. The results showed: (1) HSS was capable of promoting the proliferation of the cells, and its molecular weight was 14～20 kD; (2) the p21 ras expression was increased under HSS effect time dependently, and the effect of HSS appeared synergistic with EGF; (3) the p21 ras expression induced by HSS manifested a dose dependent manner, and the saturation effect of HSS occurred at the concentration of 100 μg/ml. Considering together with our previous report about HSS regulation on EGF receptor, these results strongly suggest that the proliferatory effect of HSS on hepatic carcinoma cells is presumedly related with EGF receptor mediated signal transduction.更多还原