【摘要】 实验在 6只成年猫上进行。将WGA HRP微量注入C5膈神经核内 ,通过逆行追踪及GABA免疫组织化学FITC荧光双重标记方法 ,研究了脑干内GABA能神经元向膈神经核的投射。结果在脑桥KF核和面神经后核周围区 (即B¨otzinger复合体 )观察到GABA HRP双标神经元。另外 ,在中缝大核、旁巨细胞外侧核及前庭神经核也观察到双标神经元。本实验结果表明 :发自上述脑干神经核团 ,特别是KF核及B¨otzinger复合体的GABA能神经元的轴突可投射到膈神经核更多还原

【Abstract】 Experiments were made on 6 adult cats. Brainstem neurons that project to the phrenic nucleus were retrogradely la~beled by microinjection of WGA HRP into phrenic nucleus. The brainstem sections were doubly processed for HRP histochemical staining with the TMB Co DAB method, and GABA immunohistochemical staining with anti GABA primary antibody and FITC conjugated immunofluorescent secondary antibody. HRP FITC double labeled neurons were observed in the pontine Klliker Fuse nucleus and areas around the retrofacial nucleus (Btzinger complex). Double labeled neurons were also observed in the raphe nucleus, the lateral paragigantocellular nucleus, and the vestibular nucleus. The results show that brainstem GABAergic neurons in these structures, especially those in the Klliker Fuse nu~cle~us and the Bt C, send axonal projections to the phrenic nucleus.更多还原