【摘要】 目的　研究肝星形细胞 (HSC)转化生长因子α(TGFα)与成纤维细胞生长因子 (aFGF)的表达。方法　分别从正常与CCl4 肝纤维化大鼠肝脏分离HSC ,提取RNA ,用RT PCR测定TGFα和aFGFmRNA。结果　正常HSC表达TGFα和aFGF ,肝纤维化时TGFα与aFGFmRNA水平均显著升高 (P <0 .0 5 )。结论　HSC自分泌TGFα和aFGF ,对肝纤维化具有重要促进作用更多还原

【Abstract】 Objective To investigate the expression of transforming growth factor α (TGFα) and acidic fibro blast growth factor (aFGF) in hepatic stellate cells (HSC) and to explore their roles in hepatic fibrogenesis. Methods Followed RNA extraction of HSC isolated from normal and CCl 4 induced fibrotic rat liver respectively, the mRNA levels of TGFα and aFGF were determined by RT PCR. Result Both TGFα and aFGF mRNA could be detected in normal HSC, however, the mRNA levels of TGFα and aFGF increased markedly in hepatic fibrosis ( P <0.05). Conclusion The autocrine TGFα and aFGF may be important regulators of HSC proliferation, and are involved in hepatic fibrogenesis. (Shanghai Med J, 2000,23∶478 480)更多还原