【摘要】 目的　研讨磷脂对HDL3 介导大鼠皮肤成纤维细胞内胆固醇流出能力的影响。方法　在卵磷脂 (PC)或鞘磷脂 (SPM )存在条件下 ,观察HDL3 介导细胞胆固醇流出量的变化、细胞内磷脂含量变化及游离胆固醇 /胆固醇酯平衡的变化。结果　①BSA组 (对照组 )、HDL3 组、PC组、SPM组、PC +HDL3 组和SPM +HDL3 组分别介导 4.70 %、31.5 5 %、7.35 %、8.0 6 %、42 .95 %和 46 .98%细胞胆固醇流出 ;②BSA、HDL3 、HDL3 +SPM和HDL3 +PC组细胞培育后胞内卵磷脂磷含量 (PC p)和鞘磷脂磷含量 (SPM p)分别为 2 0 .0 2、5 .5 6 ,17.5 6、5 .2 8,18.6 2、7.0 0和 2 2 .5 0、5 .5 2 μg/皿 ;③PC和SPM与细胞培育后 ,胞内游离胆固醇 /总胆固醇比值分别为 49.6 5和 5 9.5 7。培育前此比值为 48.6 4。结论　①PC和SPM本身无介导细胞胆固醇流出能力 ,但它们能显著提高HDL3 介导的细胞胆固醇流出能力 ,且后者强于前者 ;②随着细胞胆固醇流出 ,部分胞内PC也流出胞外 ,而胞内SPM无明显变化 ;③SPM促进细胞内胆固醇酯向游离胆固醇转化。更多还原

【Abstract】 Purpose To study the effect of phospholipid on the ability of HDL 3 mediated cholesterol efflux from rat skin fibroblasts. Methods At the present of phosphatidylcholine (PC) or sphingomyelin (SPM),to measure the changes of HDL 3 mediated cellular cholesterol efflux,cellulax phospholipid content and balance between free cholesterol and cholesteryl ester. Results ① BSA (control),HDL 3,PC,SPM,PC+HDL 3 and SPM+HDL 3 group mediated 4.70%,31.55%,7.35%,8.06%,42.95% and 46.98% of cellular cholesterol efflux from the cells respectively.② After the incubation of the cells with BSA,HDL 3,HDL 3+SPM and HDL 3+PC,the cellular phosphorous content in PC(PC p) and SPM (SPM p) were 20.02 ,5.56;17.56, 5.28 ;18.62,7.00 and 22.50,5.52 μg/plate respectively.③ After the incubation of the cell with PC and SPM,ratio of free cholesterol to total cholesterol were 49.65 and 59.57 respectively.The ratio was 48.64 before incubation. Conclusions ① PC and SPM couldn’t mediated directly cellular cholesterol efflux,but they could enhance significantly HDL 3 mediated cellular cholesterol efflux,and this ability of SPM was stronger than PC.② With cellular cholesterol efflux,a part of cellular PC went out of the cells,but the content of cellular SPM didn’t change significantly.③ SPM could induce cholesterol ester to convert into free cholesterol in the cells.更多还原