【摘要】 目的　研究转化生长因子β１（ＴＧＦβ１）对培养的大鼠肾小球系膜细胞（ＭｓＣ）基质金属蛋白酶２（ＭＭＰ２）表达的影响。方法　采用Ｌｉｐｏｆｅｃｔｉｎ将人ＴＧＦβ１基因转染培养的大鼠ＭｓＣ，经Ｎｏｒｔｈｅｒｎｂｌｏｔ鉴定其转染成功否；又分别应用Ｎｏｒｔｈｅｒｎｂｌｏｔ．Ｗｅｓｔｅｒｎｂｌｏｔ．酶谱分析法检测阳性表达人ＴＧＦβ１的ＭｓＣＭＭＰ２ｍＲＮＡ、蛋白及酶活性变化。结果　成功获得稳定过表达人ＴＧＦβ１的大鼠ＭｓＣ克隆（ＭＴＧ１），该细胞克隆的ＭＭＰ２ｍＲＮＡ．蛋白表达及其酶活性均获增强。结论　ＴＧＦβ１可增强ＭｓＣＭＭＰ２ｍＲＮＡ、蛋白表达，并提高其酶活性，这为进一步阐明ＴＧＦβ１在肾小球硬化发生机制中的作用提供了重要的实验手段和依据。更多还原

【Abstract】 Purpose To study the matrix metalloproteinase 2(MMP?2) expression on cultrured rat mesangial cells(MsC) transfected by human transforming growth factor β1(TGF?β1) gene. Methods Lipofectin method for transfecting human TGF?β1 gene into MsC;Northern blot analysis for detecting TGF?β1 mRNA level in MsC.MMP?2 mRNA,protein and enzyme activity were determined by Northern blot,Western blot,Zymography assay,respectively. Results The MsC clone (MTG1) overexpressed human TGF?β1 mRNA was successfully prepared and its expression of MMP?2 mRNA,protein and enzyme activity was increased. Results The MsC clone (MTG1) overexpressed human TGF?β1 mRNA was successfully prepared and its expression of MMP?2 mRNA,protein and enzyme activity was increased. Conclusions TGF?β1 might upregulate the expression of MMP?2 and enhance the enzymatic activity of MMP?2 on cultured MsC.It is valuable for explaining the role of TGF?β1 in the development of glomerulsclerosis.更多还原