【摘要】 将提取的玉米RNA反转录成cDNA ,以此为模板 ,合成特异性引物 ,应用多聚酶链式反应 (PCR)技术扩增出目的片段。对PCR片段直接进行序列分析 ,测定并克隆玉米的核糖体失活蛋白 (RIP)基因。序列分析表明 ,已测定的玉米RIP基因序列长为 983bp ,其中编码区长 82 8bp ,共编码有 2 75个氨基酸和一个终止密码子 ,GC含量为5 8 3%。与已发表的序列相比较其核苷酸序列及推导的氨基酸序列的同源性分别为 98 4%和 97 4%。更多还原

【Abstract】 A gene encoding maize Ribosome\|inactivating protein was amplified by means of PCR using mRNA of maize leaves as a template,and cloned into pUC19 vector.The amplified DNA sequence has been determined,which consists of 828bp and encodes 275 amino acid residues.Comparison with previously reported sequence shows 98.4% homologies in nucleotide sequence and 97.4% in amino acid sequence,respectively.更多还原