【摘要】 考察了在半连续灌注培养中WuT3杂交瘤细胞在不同灌注速率下细胞生长的动态变化,培养基中主要基质的消耗和代谢物的生成。当灌注速率D从10/d升高到20/d时,乳酸得率系数Ylac/glu降低18%,氨得率系数Yamm/gln降低40%,丙氨酸得率系数Yala/gln升高58%,甘氨酸得率系数Ygly/gln基本恒定。说明在灌注速率升高的条件下,细胞会调整代谢机制,丙酮酸和过量的谷氨酸通过转氨作用生成丙氨酸而非甘氨酸。更多还原

【Abstract】 The growth and metabolism of WuT 3 hybridoma cells were studied in semicontinuous perfusion cultures. The growth of cells,utilization of glucose,production of lactate,ammonia and monoclonal antibody were investigated. As the perfusion rate increased from 1.0/d to 2.0/d,the lactate yield coefficient from glucose Y lac/glu decreased 18%,the ammonia yield coefficient from glutamine Y amm/gln decreased 40%,the alanine yield coefficient from glutamine Y ala/gln increased 58%,the glycine yield coefficient remained almost constant. This indicate that cells would regulated their metabolic flux at higher cell density,and glutamate produced in quantity and pyruvate would turn into alanine rather than glycine by transamination. As a result,potentially toxic effects were reduced because less ammonia and lactate were produced.更多还原