【摘要】 以苜蓿丫纹夜蛾核多角体病毒 (Autographacalifornicamultinucleocapsidnuclearpolyhedrosisvirus,AcMN PV)基因组含几丁质酶基因 (chiA)的 pstIM片段为探针 ,通过Southern杂交将斜纹夜蛾核多角体病毒(Spodopteralituranuclearpolyhedrosisvirus,SpltNPV)的chiA基因定位在其基因组DNA两个相邻的XbaI片段(5 .1kb及 2 .1kb片段 )上 ,克隆了包含该基因完整序列的片段 ,并测定了它的核苷酸全序列。该基因读码框为16 95个核苷酸 ,编码 5 6 4个氨基酸的蛋白质 ,分子量为 6 2 .9kD ,在起始密码子ATG上游 - 10处有一个杆状病毒晚期启动子模体TAAG ,在终止密码子TAA下游有一个真核生物基因mRNA转录poly(A)加尾信号序列AATAAA。在SpltNPVchiA基因氨基酸序列的N端预期有由 2 3个氨基酸构成的真核生物信号肽。SpltNPVchiA基因氨基酸序列与AcMNPVchiA基因序列的同源性为 5 7%。更多还原

【Abstract】 Using AcMNPV chiA-containing fragment as a probe, the chitinase gene of Spodoptera litura nuclear polyhedrosis virus (SpltNPV) was localized in two contiguous fragments, XbaI 5.1 kb and 2.1 kb, and the intact SpltNPV chi A gene w as obtained by cloning and sequencing those two fragments. The complete open rea ding frame of the gene was 1 695 nucleotide long, encoding a putative protein of 564 amino acids with molecular weight of 62.9 kD. Sequence analysis further rev ealed that the 5′ noncoding region had a baculovirus late promoter motif TG. A polyadenylation signal, TA, was located dowmnstream of the translation sto p codon. A putative signal peptide was present at the N-terminus of the protein . This gene shares 57% homology with AcMNPV chitinase gene.更多还原