【摘要】 从皖南尖吻蝮蛇 (Agkistrodonacutus)毒腺cDNA文库中得到P I型出血毒金属蛋白酶acutolysinC的完整基因 ,长为 16 5 0bp。序列分析表明该基因编码出血毒金属蛋白酶前体分子 ,包括信号肽区、酶原区和金属蛋白酶区。信号肽区由 18个多为疏水性氨基酸残基组成 ;酶原区中含有类似于间质金属蛋白酶中保守的半胱氨酸开关序列CGVT ;金属蛋白酶区中含有典型的锌离子结合活性中心HEXXHXXGXXH序列及CIM转折 ;其后是间隔区和 3′端非编码区。金属蛋白酶区氨基酸序列与acutolysinC晶体学序列基本一致 ,与其他P I型蛇毒出血毒分子有很高的同源性更多还原

【Abstract】 A full length cDNA of 1 650 bp was amplified from the snake venom gland cDNA library of Agki strodon acutus . Analysis of the nucleotide sequence indicated that the amplified cDNA contained a complete open reading frame encoding 417 amino acid residues including signal peptide sequence, zymogen sequence and proteinase domain. The zymogen sequence contained CGVT motif that was highly conserved in almost all venom metalloproteinases. The metalloproteinase domain contained a conserved signature zinc binding motif HEXXHXXGXXH in the catalytic region and the CIM turn. It shares high similarity with the sequence of acutolysin C deduced from crystallographic data, and with other class P I snake venom hemorrhagic toxins.更多还原