In order to efficiently recover recombinant proteins, a temperature sensitive lytic system was constructed on the basis of the feature that T4 lysozyme disrupts the bacteria through cutting specific bond in the peptidoglycan layer of cell wall. This system was evaluated by constructing and introducing a low copy plasmid pSC lys (pSC101 replication origin) into E.coli . The plasmid contained a temperature sensitive T4 lysozyme (LYS ts ) gene under the control of three tandem tac promoter...
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