【摘要】 观察了雷公藤单体雷藤氯内酯醇 (T4)对雄性猕猴的抗生育作用及其可逆性。 1 2只性成熟雄性猴等分 4组 ,口服 T4剂量分别为 5 0、2 5、1 0、0μg· (kg· d) - 1,每周 6次 ,连续8周。 3个剂量组猕猴分别于给药第 8、7、5周其射出精液达到死精、无精、或严重寡精 (<1× 1 0 6 / ml)。射出精子最先出现的改变是颈体弯曲、继之头尾分离、但头部形态基本正常。给药期间各受试猴自发射精频率、电刺激射精反应、精液量、睾丸容积、血清睾酮水平及精浆果糖浓度等与对照组相比 ,没有显著差异。精浆肉毒碱浓度有变化但不甚规律。 3个剂量组猕猴分别于停药后 1 0、8、4周其射出精子密度和活率恢复到药前水平。交配试验亦证实各受试猴已恢复使雌猴受孕的能力。表明 T4对雄性猕猴具有明显的抗生育药效而且是可逆的更多还原

【Abstract】 The results of the preliminary observation on antifertility pharmacodynamics of tripchlorolide(T4) in male rhesus monkeys and reversibility of fertility after cessation of the treatment was reported.Twelve sexually mature male rhesus monkeys were randomly divided into 4 groups. T4 was adminstered orally at daily doses of 50, 25, 10, 0 μg/kg,6 times a week, for 8 consecutive weeks.Necrospermia, azoospermia or severe oligozoospermia(<1×10 6/ml) was obserueed at the end of 8th , 7th and 5th week of treatment in the treated groups respectively. Bending of midpiece and separation of the head and tail were found in the silver stained preparations of ejaculated spermatozoa after treatment, however, sperm head morphology was still almost normal. During the T4treatment period, there were no significant differences in the spontaneous ejaculatory frequency, electroejaculatory response, ejaculate weights, testicular volume, serum testosterone levels and seminal fructose concentration in treated groups as compared with the control. However, the carnitine content of seminal plasma changed nonuniformly in T4treated animals. Sperm density and viability were restored to normal levels at 10th, 8th, and 4th week after cessation of treatment in 3 treated groups respectively. The finding on reversibility of fertility was confirmed by mating test.The results showed that Tripchlorolide possesses reversible antifertirity effect in male rhesus monkeys at the tested doses.更多还原