【摘要】 确定由脂类分子介导的信号转导途径和细胞内某些信使分子的生成及改变是信号转导研究领域中的一个重要组成部分 .如确定不同磷脂酶活性的调控及细胞内不同来源的第二信使分子及其他脂类生物活性分子的生成与调节 ,成为探讨生长因子或其他许多分子的生物效应及其作用机理的重要研究内容 .为了增加人们对有关研究工作的了解及在方法上的选择 ,介绍了研究脂类代谢信号转导中广泛运用的一个基本而重要的方法———选择性标记法 ,并且以实际研究结果为例 ,说明如何运用该方法检测不同的信号传递途径和有关信号分子的生成与变化 .该法针对性强而灵活 ,重复性高 ,能有效地检测某些不同来源的信号传递分子的生成及其变化 .此外 ,对脂类代谢信号转导途径及对该途径的研究在信号转导领域的地位和意义也作了简要的介绍更多还原

【Abstract】 Determination of lipid signaling pathways and the production of some intracellular signaling molecules has been an focus of some research work in the field of signal transduction research. For example, the determination of the regulation of different phospholipase activity and the formation of intracellular second messengers and other lipid bioactive molecules from different sources is an important component in the studies on the biological effects and their mechanisms of growth factors and other molecules. Here, a method was introduced for selectively labeling different phospholipids, which has been widely used in the study of lipid signaling pathways. Experimental evidence was presented to demonstrate how to determine the different signaling pathway and the generation as well as the alteration of relevant signaling molecules. The application of this method is flexible and has a high reproducibility. It can be used to pinpoint some signaling pathways and the formation of different signaling molecules. A brief introduction about lipid signaling and its significance in the area of signal transduction was also given.更多还原