【摘要】 将对虾白斑杆状病毒的一段特异性DNA设计成分子信标探针 ,用于该病毒的PCR检测 .温度与荧光强度之间的关系表明 ,所设计探针的发夹既可以形成也可以打开 ,符合PCR对分子信标探针的要求 .结果表明 ,在PCR同时加入分子信标探针不影响PCR扩增 ,分子信标探针只能与目的DNA杂交 ,具有较高的特异性 .随着PCR循环数的增加以及含目的DNA的质粒拷贝数的增加 ,荧光强度都随之增强更多还原

【Abstract】 A molecular beacon probe was used in the detection of prawn white spot bacilliform virus (WSBV) . The probe possesses a stem and loop structure. The sequence of loop is complementary to the WSBV DNA, and the stem is formed by two complementary arm sequences which are unrelated to the WSBV DNA. The probe underwent a fluorogenic conformational change as it hybridized to the WSBV DNA. The probe could not affect the amplification , and was specific and sensitive when used in PCR. It was showed that the fluorescent intensities increased as the cycles of PCR and the number of target DNA copy increased.更多还原