【摘要】 目的探讨不同毒力的弓形由株与其致病性的关系、方法应用体外培养和动物模型观察ＲＨ、Ｂ３６、Ｆｕｋａｙａ三虫株对组织细胞的损害及其致病、致畸、致死的差异。结果Ｖｅｒｏ细胞在８ｈ内的侵袭率及分解产物脂肪酸含量以Ｂ３６株为高。胞内２４ｈ繁殖数以ＲＨ株为最多，Ｂ３６次之，Ｆｕｋａｙａ最低。此结果与虫体对细胞的损害程度、对仔鼠智力发育的影响密切相关。细胞因子激活巨噬细胞后，对弓形由抑虫或杀虫程度与ＮＯ浓度相一致。结论ＲＨ株ＮＯ浓度高，胞内虫株可被完全消灭，ＮＯ可能参与抗虫作用。更多还原

【Abstract】 Objective The comparison on virulence and pathogenicity in RH,B36 and Fukaya strain of Toxoplasma gondii was investigated. Methods By using in vitro cultivation and animal model, differences of the 3 strains, RH, B36, Fukaya, in causing tissue and cell damage, motality, criteria of physical and mental pathogenesis as well as frequency of fetal anomalies were examined. Results The parasitic invasibility to cultivated Vero-cells at 8h after infection was found the highest in B36, in which, a parallelly increased metabolic product content of fatty acids was demonstrated, the number of proliferated parasites in the host cells within 24h appeared to be the most in strain RH with a moderate in B,, and the lowest in Fukaya. The result showed a positive correlation with the degree of cell damage and the influence of intelligence in the off-springs. The intensity of parasitocidal and inhibition effect of cytokine activated M against Toxoplasma was shown to be concomitently associated with the reactive NO concentration detected in the medium. Conclusions Along with a complete elimination of the parasites in the host cells, the RH strain gave a higher NO concentration than the other 2 strains, which indicats that NO plays a role in the anti-parasite mechanism.更多还原