【摘要】 采用随机扩增多态 ＤＮＡ（ｒａｎｄｏｍ ａｍｐｌｉｆｉｅｄ ｐｏｌｙｍｏｒｐｈｉｃ ＤＮＡ，简称ＲＡＰＤ）技 术，对中国对虾的黄、渤海地理群的３２个个体遗传多样性进行了检测．使用ＯＰＩ系列 的２０个 １０ ｂｐ的寡核苷酸随机引物，对每个个体的基因组 ＤＮＡ进行扩增，结果为：１７ 个引物获得了谱带清晰且重复性好的产物，扩增片段的分子量在 ２ ０００～２００ ｂｐ之间， 共检测 １０６个位点，其中多态位点数为 ３９个，占 ３６． ８ ％；平均遗传距离为 ０． ０９４１＋ ０． ０２０ ６，有 ６７个标记（占 ６３． ２％）在整个群体的 ３２个个体间表现稳定的一致性。更多还原

【Abstract】 Genetic diversities of 32 individuals of P. chinensis in the China’s coastal waters of the Huanghai and Bohai Seas are detected by RAPD technique. 20 decamer primers of OPI are used for DNA amplification for each individual. The results show that of reproducible fingerprints 17 primers are obtained, and the bands are clear. 39 of 106 loci detected are polymorphic, amounting to 36. 8 %. Mean genetic distance is 0. 094 1 ± 0. 020 6; 68 markers (63. 2% of the total) show stable homogeneity in all of the 32 individuals.更多还原