【摘要】 根据编码人IgG1Fc(Fcγ1 )的基因序列 ,设计合成 1对与之相匹配的引物。采用RT PCR法 ,从正常人白细胞mRNA中扩增获得预期目的基因Fcγ1 70 0bp基因片段 ,成功构建PUC1 8 Fcγ1 70 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符 ;DNA测序结果与GenBank报道一致。更多还原

【Abstract】 According to the cDNA sequences which encode the human IgG1Fc, a pair of primers were designed; The expected gene (Fcγ1 700 bp ) which encode Fcγ1 was amplified by RT PCR from mRNA extracted from normal person′s leukocyte; PUC18 Fcγ1 700 recombinant cloning vector was successfully constructed, and the enzyme digestion analysis are identical to expected results. Sequences are identical to those in GenBank report.更多还原