【摘要】 应用聚丙烯酰胺凝胶电泳 (PAGE)和UVP凝胶分析仪系统 ,分析比较了中、美猪型旋毛虫成虫和幼虫的酯酶 (EST)同工酶及乳酸脱氢酶 (LDH)同工酶的带谱。结果表明 :猪型黑龙江株旋毛虫成虫和幼虫的EST同工酶酶带均为 2条 ,猪型美国株旋毛虫成虫和幼虫EST同工酶酶带分别为 3条和 2条 ;中、美猪型旋毛虫成虫的LDH同工酶无酶带 ,而幼虫则各具 1条酶带 ,且其迁移率也不同。结果提示 :来自不同地域的旋毛虫在遗传学上存在差异 ,同时 ,不同发育阶段同工酶酶谱也有明显不同。更多还原

【Abstract】 The studies on isoenzyme of esterase (EST) and dehydrogenase (LDH) in adults and larvae of Chinese and American Trichinella spiralis (T.s) by PAGE and UVP gel documentation analysis system were reported. The results showed that there were 2 bands of EST isoenzyme in adults and larvae of Chinese heilongjian T.s , respectively. There was only one band of LDH in Chinese T.s larvae. In EST analysis 3 and 2 enzymatic bands were found in adults and larvae of American T.s , respectively. No LDH isoenzyme band of were observed in adults of Chinese and American T.s . Otherwise one band was found both in Chinese larvae and American larvae, the relative mobility was also different. The results indicated that there was genetic difference in different geographical T.s strains. In addition, there was great difference in isoenzyme patterns of different developmental stages of T.s .更多还原