【摘要】 目的 :研究乌贼墨对H2 2 癌细胞内Ca2 +浓度、细胞核Ca2 +/Mg2 + ATP酶活性及c fos表达的影响。方法 :利用Fura 2 /AM荧光探针和免疫组织化学方法 ,测定乌贼墨对H2 2 癌细胞内Ca2 +浓度、细胞核Ca2 +/Mg2 + ATP酶活性及c fos表达的变化。结果 :乌贼墨使H2 2 癌细胞内Ca2 +浓度降低了 69%和79 % ,细胞核Ca2 +/Mg2 + ATP酶活性降低了 2 1%和 37% ,c fos表达明显减少。结论 :乌贼墨可能通过降低细胞内Ca2 +浓度 ,影响细胞核上Ca2 +依赖性Ca2 +/Mg2 + ATP酶活性 ,减少了Ca2 +向细胞核内的转运 ,减弱了Ca2 +对c fos表达的促进作用 ,抑制了细胞的分裂增殖。更多还原

【Abstract】 Objective:To study the effects of Sepia on the intercellular Ca 2+ concentration,nuclear Ca 2+ /Mg 2+ ATPase activities and the expression of c fos.Methods:Fluorescent probe and immunohistochemical methods were used.Results:The intracellular Ca 2+ concentration decreased by 69% and 79%,nuclear Ca 2+ /Mg 2+ ATPase activities diminished by 21% and 37%,c fos expression level decreased obviously.Conclnsion:Sepia probably reduces the amount of Ca 2+ transported into nuclei and prmotes the effect of Ca 2+ on c fos expression by diminishing Ca 2+ concentration and Ca 2+ dependent nuclear Ca 2+ /Mg 2+ ATPase activities.Thus,cellular differentiation and proliferation are inhibited.更多还原