【摘要】 采用大肠杆菌偏爱的密码子 ,合成 30个寡聚脱氧核苷酸 ,在体外进行退火和连接合成甜味蛋白thaumatincDNA基因 ,将连接得到的DNA产物克隆到质粒 pBluescript SK上 ,转化大肠杆菌DH5α细胞 ,进行诱导表达筛选 ,将筛选的克隆进行cDNA全序列测定 .更多还原

【Abstract】 The mature form of thaumatin is a sweet protein which contain 207 amino acid residues with 8 disulfide bonds, we report here the chemical synthesis and cloning of the thaumatin cDNA.After cheated with T4 DNA kinase,the 30 synthetic oligonucleotides were annealed and ligated,the ligation product was inserted into plasmid pBluescript\|SK by the sites of two restriction enzymes EcoR I and Sal I, the thaumatin cDNA had been screened through fusion expression with LacZ’ gene. The target gene was confirmed by sequencing.更多还原