【摘要】 为准确检测鸡群中减蛋综合征病毒感染情况 ,从减蛋综合征病毒 (EDS)贵州分离毒株HS- 1中提纯病毒DNA后 ,用地高辛标记制备了全基因组探针。在Dot -blot中该探针不与正常鸭胚尿囊液核酸提取物及马立克氏病病毒 (MDV)DNA发生反应 ,只与 3株EDS病毒 (国际标准毒AV - 12 7、贵州分离毒HS - 1、南京分离毒GC2 )DNA呈阳性反应 ,具有较高的特异性 ;可检测出 30pg的同源DNA ,具有较强的灵敏度。人工感染母鸡于 2 4h后即可用该探针从泄殖腔棉拭子中检测到病毒 ,能早期诊断该病的发生 ,检测阳性率为 88 89% ;并可检测到EDS病毒的隐性传播情况更多还原

【Abstract】 The Digoxigenin(Dig) probe with HS-1 whole genomic DNA was developed by purified EDS virus. In the hybridization, the probe reacted only with 3 strains of EDS virus, but not with Marek’s disease virus, normal duck embryo allantoic fluid DNA samples. So it was of high specificity and sensitivity and 30 pg DNA could be detected in experiment. By Dot-bolt hybridization the probe had detected virus’s DNA in cloacal swabs for early diagnosis, and the positive rate of samples was 88.89% in artificial infected hens. In addition it could be used in EDS recessive infection.更多还原