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用毛细管电泳测定发酵液中柠檬酸的含量
Determination of Citric Acid in Fermentation Liquor by Capillary Electrophoresis
【摘要】 本文研究了毛细管电泳在检测柠檬酸发酵液中的应用 ,比较了经炭吸附处理与不经炭吸附处理两种处理方法对毛细管电泳测定发酵液中柠檬酸含量实验的影响。结果表明 ,发酵液中色素、糖等杂质对柠檬酸的电泳测定无干扰 ;炭粉吸附色素、糖等杂质的同时也吸附了部分柠檬酸 ,使测定结果降低 ;柠檬酸发酵液可以只经过抽滤即可用毛细管电泳进行测定 ,实验操作简便迅速 ,结果可靠。为工业发酵液的检测提供了一种新的可行的方法。
【Abstract】 In this paper,the application of capillary electrophoresis in determination of citric acid in fermentation liquor and the effect of carbon powder absorbing to the results were studied.It was found that the impurity in fermentation liquor,such as coloring matter and sugar didn’t affect the determination results and carbon powedr could weakly absorb citric acid together with coloring matter and sugar,which made the result lower.So citric acid in fermentation liquor can be directly determined by capillary electrophoresis only after the fermentation liquor was filtered.The method is simple,quick and reliable.It provided a new and reliable method for the fermentation liquor determination.
- 【文献出处】 山东化工 ,Shandong Chemical Lndustry , 编辑部邮箱 ,2000年06期
- 【分类号】O657.1
- 【被引频次】8
- 【下载频次】237