【摘要】 目的 :建立一种用饱和盐水比浊法测定血浆纤维蛋白原的方法。方法 :用饱和盐水使血浆纤维蛋白原产生浊度 ,通过比浊测定血浆纤维蛋白原浓度 ,并进行方法学评价和对照试验。结果 :该法线性好 y =2 0 .0 6 73x - 0 .0 2 2 4,r=0 .999,批内重复试验CV =2 .5 % ,批间重复试验CV =3.0 % ,平均干扰率 <5 % ,回收率为 97%。与双缩脲法比较r =0 .91 94,y =0 .9781x - 0 .40 0 2有较高的一致性。 结论 :本方法简便、易掌握 ,测定结果能满足临床要求 ,不失为血浆纤维蛋白原测定的可选方法更多还原

【Abstract】 Objective:To establish one method for determining fibrinogen in plasma by saturated salt solution turbidimetry.Method:We made the fibrinogen turbidty with saturated salt solution and determined the fibrinogen by turbidimetery.Then did methodological evaluation and comparison.Result:The linearity was very good.y=20.0673x 0.0224. r =0.9999.The within run cv was 2.5%.The between run cv was 3.0%.Average interfere rate was lower than 5%.The recovery rate was 97%.Compared turbidimetry with Biuret,We established the following regressive equation:y=0.9781x 0.4002. r =0.9194.Conclusion:Turbidimetry is simpler and easy to master.The determined results are satisfactory.Therefore,It can be used as one method for determining fibrinogen in plasma in clinical.更多还原