【摘要】 :以海捕中国对虾为材料 ,利用多聚酶链反应 (PCR)技术和核酸探针技术 ,对从中国对虾杆状病毒核酸随机文库中筛选的 4个片段 (大小分别为 80 0 ,1 1 0 0 ,1 5 0 0 ,2 5 0 0bp)用地高辛标记作为探针 ,进行斑点杂交 ,检测对虾杆状病毒。结果表明海捕中国对虾的鳃、中肠、肝胰腺、卵巢等组织检测为阳性 ,肌肉组织为阴性。实验表明中国对虾杆状病毒存在垂直传播的可能性更多还原

【Abstract】 Wild caught Penaeus chinensis was detected for baculovirus by Polymerase Chain Reaction(PCR) and dot blot hybridization using DIG labeled Penaeus chinensis Baculovirus (PCBV) fragment of nuclein lengthened 800,1 100,1 500 and 2 500bp. Positive reaction was found in gills,hepatopancreas,midgut and ovaries ,but muscular tissue was negative. Our results demonstrated that the ovaries of shrimps were infected by the baculovirus that implied the virus may be transmitted vertically from parent to young generation.更多还原