【摘要】 为寻找恶性疟原虫基因工程多表位疫苗保护性评价的模型动物,利用鼠约氏疟原虫（Plasmodium　yoelii）及伯氏疟原虫小鼠（Phasmodium　bergher）模型对本实验室构建的恶性疟原虫（Plasmodium　falciparum）多表位基因工程候选疫苗进行了体内保护性及免疫模式试验。结果在约氏疟原虫,先用该疫苗基因的大肠杆菌表达蛋白免疫、后用含同一基因的重组痘苗病毒加强免疫组与野生型痘苗病毒免疫组及空白对照相比,小鼠的死亡时间延长（P<0.01）。结果初步显示,约氏疟原虫鼠模可用作该基因工程多表位恶性疟原虫候选疫苗的保护性评价。更多还原

【Abstract】 To find the suitable animal model for the vaccine assessment of Plasrnodium falciparum, Plasrnodiumyoelii and Piasrnodium bergher, mice were used to evaluate the protection for a multi-epitope candidate vaccine of Plasmodium falciparum. The results showed that the mice of the group of firstly immunized with the protein expressed in E. colt , boosted with the recombinant vaccinia virus with the same epitopes survived longer than that of groups of immunized with the wild vaccinia virus and blank control mice (P<0.01). This indicats that Plasmodium yoelii can be used as models for the evaluation of the protective effect of multi-epitope vaccine candidate of Plasmodium falciparum.更多还原