【摘要】 为了制备抗华支睾吸虫基因工程抗体,本文从感染华支睾吸虫患者的淋巴细胞中提取总RNA,逆转录成cDNA。用相应的引物进行PCR,扩增出约700bp的重链Fd段和轻链κ、λ基因,经Xho　I和Spe　I,SacI和 Xba　I双酶切后,分别和质粒载体pComb3连接,再经电穿孔转化大肠杆菌XL 1-blue菌株,将轻链和重链Fd基因先后克隆人pComb3中,成功地构建了抗华支睾吸虫Fab段抗体基因的表达载体,为进一步构建噬菌体抗体库奠定基础。更多还原

【Abstract】 The total RNA extracted from PBLs of 16 patients infected with Clonorchis sincnsis was reverse transcripted, and heavy chain Fd gene and light chain genes of the imonunoglobulin were amplified by PCR using primers scanning γ1 Fd and κ、λchain. After digestion with Xhol + Spel and SacI + Xbal, the amplified Fd and light chain fragments were cloned into phagemid pComb3 and electrotransinfected into competent E. coli XLl-blue respectively. By then, a recombinant expression vector bearing antibody Fab genes against C. sinensis was constructed. Further screening, identification and sequencing are undertaking.更多还原