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日本血吸虫未知基因JAYL0230全长cDNA的克隆和测序分析
Choning and Sequencing of a Novel Gene JAYL0230 of Schistosoma japonicum
【摘要】 为获取日本血吸虫未知基因全长cDNA,通过EST序列的同源性比较,发现日本血吸虫未知基因序列,将未知基因克隆的cDNA插入片段克隆到载体pBluscript(KS),并对其进行序列测定和序列分析。结果获得日本血吸虫未知基因JAYL0230的克隆,序列分析结果提示该cDNA序列含有一个402bp的完整阅读框序列。
【Abstract】 To obtain and analyse the complete sequence of a novel gene of Schistosoma japonicum . Methods: A novel gene of Schistosoma japonicum was found by homogenous analysis and was cloned into Blucript(KS), sequence it and analyse it with bioinformatic software. Results: A recombinant plasmid JAYL0230-Bliuscript(KS) was obtained. It contains a complete open reading frame(ORF), and maybe has putative function. Conclusion: Expressed sequence tags strategy and homogenous analysis are efficient to find novel genes.
【关键词】 日本血吸虫;
未知基因;
克隆;
全长cDNA;
【Key words】 Schistosma japonicum; novel gene; clone; full length sequence;
【Key words】 Schistosma japonicum; novel gene; clone; full length sequence;
【基金】 本课题获得广东省“211工程”重点学科建设课题(98169);国家自然科学基金(30070683)的资助.
- 【文献出处】 广东寄生虫学会年报 , 编辑部邮箱 ,2000年00期
- 【分类号】R392
- 【被引频次】2
- 【下载频次】74