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树突状细胞在混合淋巴细胞反应中辅佐功能的研究
Assisted Functional Study in Dendritic Cell in Mixed Lymphocyte Reaction
【摘要】 目的 :研究树突状细胞在混合淋巴细胞反应中的辅佐功能。方法 :应用MTT法检测人外周血分离培养的DCs在同种异体的混合淋巴细胞反应中激发同种T淋巴细胞增殖的能力。结果 :只需少量DCs ,即可强烈激发同种T淋巴细胞增殖。结论 :DCs是T淋巴细胞增殖反应中的重要辅佐细胞 ,具有良好的刺激同种T淋巴细胞增殖的能力
【Abstract】 Obgective:To investigate the assisted function of dendritc cells(DCs)in mixed lymphocyte reaction.Methods:The proliferation ability of allogeneic T cells stimulated by dendritic cells from human periphral blood were observed by MTT method.Results:Only a little number of dendritic cells can stimulate the proliferation of allogeneic T cells efficiently.Conclusions:DCs are important assisted cells in T cells proliferation. They are potent stimulators in allogeneic mixed lymphocyte reaction.
【关键词】 树突状细胞;
辅佐功能;
MTT法;
MLR;
【Key words】 dendritie cell; assisted ability; MTT method; mixed lymphocyte reaction;
【Key words】 dendritie cell; assisted ability; MTT method; mixed lymphocyte reaction;
【基金】 山东省卫生厅资助项目!(982030401)
- 【文献出处】 肿瘤防治杂志 ,JOURNAL OF QILU ONCOLOGY , 编辑部邮箱 ,2000年04期
- 【分类号】R730.2
- 【被引频次】7
- 【下载频次】202