【摘要】 目的 :观察了潘生丁对KB细胞株多药耐药的逆转作用并对其作用机制进行探讨。方法 :使用MTT法检测比较KB母细胞和耐药细胞株对长春新碱 (VCR)的药物敏感度并观察潘生丁对耐药的逆转程度 ;用3H标记的VCR检测细胞内药物浓度的变化。结果 :潘生丁作用下 ,KB母细胞和耐药细胞株的药物敏感度均有增高并呈剂量相关关系 ,有MRP表达的耐药细胞株对VCR的敏感度比母细胞株高 17倍。母细胞株和耐药细胞株细胞内的药物蓄积均有明显增加。结论 :潘生丁能通过增加细胞内的VCR蓄积 ,显著提高KB细胞对药物的敏感度 ;耐药细胞株与母细胞株相比 ,有较大程度的逆转 ,可能与MRP的表达有关 ,有待进一步探讨更多还原

【Abstract】 Objective:This study is aim at investigating the reversal effect of dipyridamole and its mechanism. Methods:The enhancement of chemosensitity of KB cells to vincristine(VCR) by dipyridamole in different concentrations was analysed with MTT method.The accumulation of 33HVCR in cells is measured.Results:The chemosensitivity to vincristine is enhanced in a dosedependent way and 3 HVCR accumulation is raised by dipyridamole in both cell lines while there is a higher reversal effect in MRP expressing resistant cells.Conclusions:Dipyridamole can increase VCR accumulation and reverse the resistance to VCR in KB cells.The effect of MRP might be involved.更多还原