【摘要】 采用现代核磁共振技术 ,通过分析灌胃给药 0 .2 ,2 .0 ,1 0 ,2 0mg/kg剂量的La(NO3) 36个月后大鼠血清中某些内源性化合物的变化研究了稀土化合物在动物体内的作用情况及长期毒性 ,同时对各剂量组三个月后大鼠尿液的1 HNMR谱图进行了分析 ,并通过血液中一些重要生化指标的测定对结果进行了验证 .体液中氨基酸、乳酸、N 氧三甲胺、3 羟丁酸、葡萄糖、尿素、柠檬酸等重要内源化合物的核磁共振谱峰发生了明显的变化 ,意味着动物体内的代谢出现异常 .结果表明高剂量稀土的引入可能使动物肾脏和肝脏均受到损害 ,且受损程度随稀土剂量的增高而渐趋严重更多还原

【Abstract】 H NMR spectroscopy has been used to assess chronic toxicological effects of rare earth. Male Wistar rats have been administrated orally with La(NO\-3)\-3 at doses of 0.2,2.0,10 20mg/kg body weight for six months. The analysis for \{\}\+1H NMR spectra of rats serum and rats urine after dosing rare earth was reported. The \{\}\+1H NMR variation of numerous low \%Mr\% metabolites, including ketone bodies, lactate, glucose, urea, citrate and various amino acids, provided a sensitive measure of La\+\{3+\} induced renal and liver damage and information on abnormal patterns of metablite excretion. The assay of some biochemical indexes in plasma supported NMR results. \{\}\+1H NMR techniques could be applied to clinical diagnosis of abnormal metabolism of the inborn disease and the investigation of the toxicological effects of other drugs.更多还原