节点文献

微量滴度酶标板法测定棉蚜(Aphis gossypii Glover)酯酶特性与有机磷抗性的关系

A 96-Well Microplate Reader for Measuring Characterization of Esterase and Insecticide Resistance in the Cotton Aphids (Aphis gossypii Glover)

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 谢佳燕何凤琴冯国蕾

【Author】 Xie Jiayan He Fengqin Feng Guolei(Institute of Zoology, Academia Sinica, State Key Laboratory of IntegratedManagement of Pest Insects and Rodents, Beijing 100080)

【机构】 中国科学院动物研究所!农业虫鼠害综合治理国家重点实验室北京100080

【摘要】 通过生物测定表明高密棉蚜对有机磷的抗性高于北京棉蚜 ,用紫外分光光度计比色法 (A法 )及微量滴度酶标板法 (B法 )测定高密棉蚜及北京棉蚜的 α-乙酸萘酯酯酶活力和α-乙酸萘酯酯酶动力学。北京棉蚜和高密棉蚜的 α- NA酯酶活力分别为 2 .2 3、4 .4 8(A法 )和1.13、3.30 (B法 ) μmol· mg-1pro.· min-1,高密、北京棉蚜的酶活之比为 2 .0 0 (A法 )、2 .92 (B法 ) ;北京棉蚜、高密棉蚜的 K m值分别为 :6 .0 6× 10 -5、7.51× 10 -5(A法 )和 7.6 6× 10 -5、8.87× 10 -5(B法 ) mol· L-1,Vmax 值为 2 .53、 5.82 (A 法 )和 1.2 8、 3.6 1(B法 )μmol· mg-1· min-1。比较紫外分光光度计比色法及微量滴度酶标板法的测定结果 ,表明微量滴度酶标板法的测定结果是可靠的。

【Abstract】 The bioassay showed that Gaomi cotton aphid had higher resistance to organophosphate insecticides than Beijing cotton aphid. Using two approaches, a conventional spectrophotometer (method A) and a 96 well microplate reader method (method B),to detect general α naphthyl acetate(α NA) esterase activity and kinetics of α NA esterase of Gaomi and Beijing cotton aphid. The α NA esterase activity of Beijing and Gaomi cotton aphid were 2.23 and 4.48 (method A),1.28 and 3.30 μmol·mg -1 protein·min -1 (method B),respectively; The α NA esterase activity ratio of G/B were 2.00 and 2.56; K m of α NA esterase in Beijing and Gaomi cotton aphid were 6.06×10 -5 and 7.51×10 -5 (method A),7.66×10 -5 and 8.87×10 -5 (method B) mol·L -1 , respectively; V max were 2.53 and 5.82 (method A),1.28 and 3.61(method B) μmol·mg -1 protein·min -1 , respectively. Data obtained from the 96 well microplate reader were compared to those obtained, under similar conditions, in a conventional spectrophotometer. The approach of using the 96 well microplate reader should be generally applicable to monitor pest resistance to insecticide.

【基金】 国家“九五”科技攻关项目;国家重点自然科学基金!(批准号 :396 30 2 10 )
  • 【文献出处】 农药学学报 ,Chinese Journal of Pesticide Science , 编辑部邮箱 ,2000年01期
  • 【分类号】S433
  • 【被引频次】13
  • 【下载频次】83
节点文献中: 

本文链接的文献网络图示:

本文的引文网络