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闹羊花素-III对菜青虫神经系统的影响
Effects of Rhodojaponin-III on Nervous System of Larvae of Imported Cabbage Worm, Pieris rapae L.
【摘要】 以闹羊花素 - III处理 5龄菜青虫幼虫 ,试虫乙酰胆碱酯酶 (ACh E)的活体活性和离体活性均与对照无明显差异 ,乙酰胆碱 (ACh)含量显著降低 ;对 Na+ - K+ - ATP酶、Ca2 + -Mg2 + - ATP酶比活力有可逆性激活作用 ,处理后 2 4 h比活力提高 ,尤以低剂量 1μg·虫 -1处理的比活力显著提高 ;处理后 6 0 h,以 1~ 3μg·虫 -1处理的比活力恢复正常 ,以 5~ 10μg·虫 -1处理的比活力显著低于对照。
【Abstract】 Effects of Rhodojaponin III(Abbr.R III) on nervous system of 5th instar larvae of imported cabbage worm, Pieris rapae L. under laboratory conditions were reported. Results showed that activities of acetylcholinesterase (AChE), both in vitro and in vivio were not significantly different from that of the control, while contents of acetylcholine (ACh) were remarkably decreased. R III had reversible activated effects on Na + K + ATPase and Ca 2+ Mg 2+ ATPase activities. R III activated all ATPase activities when treated after 24h. A low dosage of 1 μg of R III per larva significantly activated all ATPase activities over the control. It’s observed that worms treated after 60h by 1~3μg R III per larva recovered. While they were fed with 5~10μg R III per larva, ATPase activities decreased to levels less than that of the control.
【Key words】 Rhodojaponin III; Pieris rapae L., Acetylcholinesterase (AChE); Acetylcholine(ACh); Na + K + ATPase; Ca 2+ Mg 2+ ATPase;
- 【文献出处】 农药学学报 ,Chinese Journal of Pesticide Science , 编辑部邮箱 ,2000年01期
- 【分类号】S481
- 【被引频次】18
- 【下载频次】199