【摘要】 为了实现外源蛋白质在动物乳腺中特异性表达，采用ＰＣＲ法扩增了ＢＬＧ ３’端含第６，７外显子及ｐｏｌｙ（Ａ）加尾信号下游约２００ｂｐ的０．８７ｋｂ片断。以山羊ＢＬＧ ５’的３．２ ｋｂ（含第１，２外显子）启动子，山羊ＢＬＧ ３’０．８７ｋｂ序列，鸡α－珠蛋白基因簇。基因上游的－２．４～－５．３ｋｂＨｉｎｄⅢ序列，绿色荧光蛋白ＧＦＰ及原核载体ｐＧＥＭ－７ｚｆ构建了ｐＧＥＭ－７ｚｆ－ＧＦＰ－ＭＡＲ－５’ＢＬＧ－３’ＢＬＧ的乳腺特异性表达载体，将外源基因插入ＢＬＧ ５’端ＥｃｏＲＩ位点，或许可能实现其在乳腺中位置独立性表达。更多还原

【Abstract】 To express valuable proteins in animal mammary glands, a 0.87 kb beta-lactoglobulin gene 3’ flanking sequence was amplified by PCR. Goat beta-lactoglobulin gene 3.2 kb promoter, 0.87 kb 3’ flanking sequence, 2.9 kb HindⅢ fragment of up stream-2.4--5.3 chicken a-globin group π gene, GFP gene, and pGEM-7zf were used to construct a vector. A foreign gene could be inserted at EcoR I site of the vector to realize the expression independent of position in transgenic animals.更多还原